Galectin-9 and Attenuation of B-Cell Signalling
Prof. Michael Corrin¹, Dr. Bebhinn Treanor² (content expert)
Undergraduate immunology students
RCSB Protein Data Bank, Adobe Illustrator
This is a molecular biology illustration portraying the role of an adapter protein Galectin-9 in B-Cell immunity. The premise of this research topic was provided by Dr. Bebhinn Treanor at University of Toronto. Molecular structures of proteins involved were referenced from Protein Data Bank (PDB); inter- and intra-cellular details were referenced from histological publications. Illustrator rendering was based on detailed pencil draft.
¹Biomedical Communications, University of Toronto, ²Department of Immunology, University of Toronto
Immunological pathways span multiple magnification scales and are often challenging to communicate to non-specialists or lay audience. With this project, I aimed to create a compelling, understandable visualization of a potential immunological pathway from ongoing research.
2. Background Research
This project was done in collaboration with Dr. Bebhinn Treanor from University of Toronto, who conducted research as portrayed in the piece. Existing publications were investigated alongside information interviews with Dr. Treanor and her lab to consolidate content into a coherent narrative. Protein structures were derived from existing or homologous structural biology data from Protein Data Bank (PDB), visually simplified, and verified by Dr. Treanor.
3. Layout Design
The final deliverable piece for this project was to be printable as a tabloid double-page spread, 11'' x 17''. For establishing layout, factors like magnification scale, narrative flow, perspective, and salience were taken into consideration. A large inset was utilized for a major magnification scale change. A refined tonal pencil draft was created as a visual template for the final rendering.
4. Vector-based Rendering
As a technical exercise, the final rendering was carried out as completely vector-based graphics in Adobe Illustrator. As such, a wide variety of vector graphics effects and techniques were employed to enhance visual fidelity.
Gasparrini, F., Feest C., Bruckbauer, A., Matilla, P.K., Muller, J., Nitschke, L., Bray D., andF.D. Facundo. "Nanoscale organization and dynamics of the siglec CD22 cooperate with the cytoskeleton in restraining BCR signalling." EMBO J. 35 (2016): 258-280.
Hermiston, M. L., J. Zikherman and J. W. Zhu. "CD45, CD148, and Lyp/Pep: critical phosphatases regulating Src family kinase signaling networks in immune cells." Immunol Rev 228 (2009): 288-311.
Krstic, R.V. Human Microscopic Anatomy 2nd printing. Plate 43-44: Splenic cords and splenic sinusoids. Berlin: Springer-Verlag, 1994.
Treanor, B.. "B-cell receptor: from resting state to activate." Immunology 136 (2012): 21-27.
Uniprot. “PTPRC – Receptor-type Tyrosine-protein Phosphatase C Precursor – Homo sapiens (Human).” Last modified November 2, 2016.
Uniprot. “CD22 – B-Cell receptor CD22 Precursor – Homo sapiens (Human).” Last modified November 2, 2016.
Wollscheid, B., D. Bausch-Fluck, C. Henderson, R. O'Brien, M. Bibel, R. Schiess, R. Aebersold and J. D. Watts. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Nat Biotechnol 27 (2009): 378-386.